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Additionally, the brink volume of CYC9 or CRK12 demanded for each of these functions could possibly be different, making sure that depletion of CYC9 or CRK12 might be adequate to disrupt cytokinesis or endocytosis, although not the two.Lister 427 cells. Trypanosomes had been transfected with each plasmid separately, then subjected to a 2nd transfec